1. This guide contains a minor spoilers.
2. To unlock each Gauntlet, we have to beat the game twice ON THE SAME SAVE FILE, because in order to unlock all the Gauntlets, we have to beat some of the bosses which are locked after choosing either option during our confrontation with Owl, when we have to choose to stay loyal to Kuro or not. Because of that, I suggest completing the game as follows:
- during our first playthrough, choose ,,Break the iron code, stay loyal to Kuro''
- after finishing the game, on New Game Plus, choose ,,Obey the iron code, forsake Kuro''.
3. There are NO STEAM ACHIEVEMENTS for unlocking and completing the Gauntlets. However, there are some rewards which will be listed further into the guide.

In the game, there are 4 Gauntlets of Strength (we will unlock 3 of them rather easily. The 4th one, however, is more unique and is unlocked differently):

1. Gauntlet of Strength: Divine Heir
2. Gauntlet of Strength: Severance
3. Gauntlet of Strength: Shura
4. Gauntlet of Strength: Mortal Journey

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To unlock each of the Gauntlets, we have to beat the following bosses:

1. Gauntlet of Strength: Divine Heir
- Lady Butterfly
- Isshin (Isshin, The Sword Saint (after choosing to stay loyal to Kuro) OR Isshin Ashina (after choosing to forsake Kuro))

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2. Gauntlet of Strength: Severance
- Isshin, The Sword Saint

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3. Gauntlet of Strength: Shura
- Isshin Ashina
- Demon of Hatred
- Owl (Father)

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4. Gauntlet of Strength: Mortal Journey (This Gauntlet is different from the others. In order to unlock it, we have to beat the final bosses in all 3 other Gauntlets)

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FIRST PLAYTHROUGH - STAYING LOYAL TO KURO:
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Lady Butterfly - she can be found at the end of The Hirata Estate - the area of The First Memory which can be accessed after receiving a Young Lord's Bell Charm from Inosuke's Mother when exploring Ashina Outskirts (she's hard to miss - she can be found in one of the destroyed houses).
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Owl (Father) - the hardest one to get to (and the most missable). Remember to complete the following points BEFORE FIGHTING the final boss of The Fountainhead Palace The Divine Dragon . After defeating Great Shinobi Owl :

1. Go down and talk to Kuro;
2. Go behind the wall Kuro is facing and eavesdrop on Kuro and Emma;
3. Rest by the idol and confront Emma (she can be found upstairs);
4. Talk to her, agree to her plan and talk to her further until she gives you Tomoe's Note;
5. Teleport to the ,,Old Grave'' idol in ,,Ashina Castle'' area;
6. Go through the gates on the left, where you will find Emma standing in front of the graves;
7. Talk to her;
8. Teleport to Dilapidated Temple;
9. Go behind the temple and eavesdrop on Emma and the Sculptor;
10. Confront Emma to reveal her secret (you will receive Father's Bell Charm );
11. Use it to access The Second Memory (WARNING! Activating the second memory will replace some of the areas from The First Memory (the one with Lady Butterfly). If you still want to clear the First Memory, do so before activating Father's Bell Charm, as after that there will be no possibility to access The First Memory;
12. Progress through the area until you come across Lady Butterfly arena, but instead of her, there will be Owl (Father) waiting to battle you.
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Demon of Hatred - After defeating The Divine Dragon at the end of the Fountainhead Palace, he can be found roaming in Ashina Outskirts, where we fought MYYY NAAAAAME IS GYOUBU MASATAKA ONIWAAA...sorry, I couldn't resist (Gyoubu Oniwa)
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Isshin, the Sword Saint - He can be found at the end of the game (after defeating The Divine Dragon ), in Ashina Reservoir, where we fought Genichiro Ashina and lost our arm at the beginning of the game.

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SECOND PLAYTHROUGH - ABANDONING KURO:
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Isshin Ashina - he can be fought after defeating Emma, the Gentle Blade right after choosing to abandon Kuro during our confrontation with Owl.
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Gauntlet of Strength: Divine Heir

BOSSES:

1. Gyoubu Oniwa
2. Lady Butterfly
3. Inner Genichiro

REWARDS:

- Skill: Sakura Dance
- Memory: Inner Genichiro

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Gauntlet of Strength: Severance:

BOSSES:

1. Guardian Ape
2. Great Shinobi - Owl
3. True Monk
4. Genichiro, Way of Tomoe
5. Inner Isshin

REWARDS:

- Another's Memory: Tengu
- Memory: Inner Isshin

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Gauntlet of Strength: Shura:

BOSSES:

1. Emma, The Gentle Blade
2. Isshin Ashina
3. Demon of Hatred
4. Inner Father

REWARDS:

- Another's Memory: Shura
- Memory: Inner Father

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Gauntlet of Strength: Mortal Journey:

BOSSES:

1. Gyoubu Oniwa
2. Lady Butterfly
3. Genichiro Ashina
4. Guardian Ape
5. Corrupted Monk
6. Great Shinobi - Owl
7. Headless Ape
8. Emma, The Gentle Blade
9. Isshin Ashina
10. True Monk
11. Owl (Father)
12. Demon of Hatred
13. Genichiro, Way of Tomoe
14. Isshin, the Sword Saint
15. Inner Genichiro
16. Inner Father
17. Inner Isshin

REWARDS:
- None (pure satisfaction for completing the Gauntlet)

Thank You for following my guide. I hope it helped you :)